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Infrared data analysis can involve peak identification, where each peak is assigned to a specific functional group or bond type. Specific analysis projects include: peak identification, peak comparison, semi-quantitative peak analysis, peak deconvolution, protein secondary structure analysis,two-dimensional infrared analysis, and other types of analyses.
Infrared testing generally involves methods such as potassium bromide (KBr) pellet method, ATR (Attenuated Total Reflection), and liquid sample cell method.
Transmittance spectra tend to highlight smaller peaks, which may sometimes make it easier to visually assess a sample. Absorption spectra have a linear relationship with concentration (while transmittance spectra do not). This makes absorbance suitable for quantitative analysis, spectral subtraction, and other operations. In peak refinement analysis, absorbance is often used due to the linearity of the spectral feature.
In infrared testing, absorbance and transmittance data can be converted using .
Materials Field:
Analyze polymers, rubbers, coatings and other materials to determine the components of additives in the materials, the degree of aging, and the intermolecular interactions.
Pharmaceutical Industry:
Identify the active ingredients of drugs, monitor the drug synthesis process, check the changes in drug crystal forms, and ensure the quality and stability of drugs.
Environmental Monitoring:
Detect environmental pollutants, such as volatile organic compounds in the atmosphere and organic pollutants in water, to assist in environmental quality assessment.
Food and Agriculture:
Identify additives and preservatives in food, analyze the quality of agricultural products, and detect the organic functional groups in pesticide residues.
The sample may absorb moisture from the air, or the KBr may not have been properly dried before testing.
ATR (Attenuated Total Reflection) involves placing the sample on an ATR crystal. The infrared light undergoes attenuated total reflection within the ATR crystal before reaching the detector. This method is commonly used for bulk, thin film, liquid, paste, gel, powder, and flexible polymer samples. It is easy to operate and avoids the interference of water peaks caused by KBr absorbing moisture.
Fourier Transform Infrared (FTIR) spectroscopy, as a highly efficient and precise analytical technique, can identify the functional groups of organic and inorganic compounds, analyze their structures, and conduct qualitative and quantitative analysis by analyzing the absorption peaks of infrared light by substances.This technique is widely used in material science, chemistry, biology, environmental science, and other fields for the identification and quantitative analysis of compounds.